Associate Research Scientist - Organoid
Pandorum Technologies
Organoid and Spheroid Platform Development
- Develop, optimize, and characterize tissue-specific 3D spheroid and organoid models derived from primary cells, stem cells, and relevant human cell sources.
- Establish reproducible protocols for organoid generation, expansion, maintenance, differentiation, and cryopreservation.
- Design and implement disease-relevant in vitro models for evaluating regenerative and therapeutic interventions.
- Develop novel 3D culture methodologies and improve platform robustness, scalability, and reproducibility.
- Perform phenotypic, molecular, and functional characterization of organoids and spheroids.
- Develop and validate medium- to high-throughput cell-based assays for biomarker quantification and therapeutic efficacy assessment.
- Design and execute functional assays related to tissue regeneration, inflammation, fibrosis, angiogenesis, wound healing, and cellular function.
- Optimize assay workflows and establish qualification criteria for reproducibility and robustness.
- Support assay transfer, standardization, and technology implementation across teams.
- Perform mammalian cell culture, primary cell culture, stem cell culture, and differentiation studies.
- Conduct molecular and biochemical analyses including:
- ELISA and multiplex cytokine assays
- RNA isolation and purification
- RT-qPCR and gene expression analysis
- Protein quantification assays
- Western blotting
- Immunocytochemistry and immunofluorescence
- Flow cytometry (preferred)
- Support sample preparation and analysis for microscopy-based studies including phase contrast, fluorescence, confocal, and high-content imaging.
- Perform tissue processing, fixation, paraffin embedding, cryo-embedding, microtomy, and cryosectioning of organoids, spheroids, and engineered tissue constructs.
- Conduct histological characterization using H&E and relevant special stains.
- Perform immunohistochemistry (IHC) and immunofluorescence (IF) for tissue-specific marker analysis.
- Analyze tissue architecture, differentiation status, cellular organization, and biomarker expression in 3D tissue models.
- Support quantitative image analysis and histology assessments.
- Design experiments and perform data acquisition, processing, statistical analysis, and interpretation.
- Prepare technical reports, study summaries, scientific presentations, and publication-quality figures.
- Maintain accurate experimental records and documentation.
- Contribute to scientific publications, patents, grant proposals, and regulatory documentation.
- Independently design and execute R&D projects aligned with organizational goals.
- Mentor and train Research Associates and junior scientists.
- Coordinate activities across multidisciplinary teams including biology, process development, analytics, translational research, and product development.
- Participate in scientific discussions, project reviews, and strategic planning initiatives.
- Develop, optimize, and maintain SOPs, protocols, and experimental workflows.
- Ensure compliance with laboratory safety practices and internal quality standards.
- Support continuous improvement initiatives and implementation of best laboratory practices.
- PhD in Cell Biology, Molecular Biology, Biotechnology, Biochemistry, Regenerative Medicine, Tissue Engineering, Biomedical Sciences, or a related field.
- Minimum 1–2 years of relevant postdoctoral and/or industrial research experience.
- Prior experience in organoid, spheroid, tissue engineering, or disease modeling platforms is preferred.
- Experience working in an industry R&D environment is preferred.
- Extensive hands-on experience in:
- 3D organoid and/or spheroid culture systems
- Mammalian, primary, and stem cell culture
- Stem cell differentiation and tissue model development
- Disease modeling and cell-based functional assays
- Histology workflows including tissue processing, embedding, microtomy, cryosectioning, H&E staining, IHC, and IF
- RT-qPCR, ELISA, immunostaining, and molecular biology techniques
- Confocal and fluorescence microscopy
- Experimental design, data analysis, and statistical interpretation
- Experience in regenerative medicine, tissue engineering, and translational research.
- Experience with high-content imaging systems and image analysis software.
- Familiarity with biomarker discovery and mechanism-of-action studies.
- Experience supporting preclinical research programs.
- Demonstrated scientific productivity through publications, patents, technology development, or grant participation.
- Strong scientific curiosity and innovation-driven mindset.
- Excellent experimental troubleshooting and problem-solving abilities.
- Ability to independently manage research projects and timelines.
- Strong communication, presentation, documentation, and mentoring skills.
- Ability to work effectively within multidisciplinary teams in a fast-paced research environment.